Review

human csf thbs1 (Boster Bio)

Boster Bio is a verified supplier

Boster Bio manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Boster Bio human csf thbs1

Figure 3. Intercellular communication networks in and around mLVs. A) Spatial visualization of immune cell inﬁltration based on mLECs distribution. B) Outgoing and incoming signal patterns among all meningeal cells. C) THBS signaling pathway network among all cell types. D) Spatial visualization of <t>THBS1-CD47</t> L-R pair distribution by stLearn. E) Volcano plots showing that THBS1 is one of the upregulated DEGs in the SAH group compared to the NPH group identiﬁed by mass spectrometry. F) Quantiﬁcation of THBS1, THBS2, and THBS4 expression in CSF samples from SAH patients compared to those from NPH patients by ELISA assay, *** p < 0.001 versus NPH by paired two-tailed Student’s t-test. G) Representative confocal images of mLVs region of the negative control (NC) group and recombinant THBS1 protein treatment (rTHBS1) group. An enlarged view of TS and SSS is shown on the right in each group. Lyve1 (blue), Beads (green), and CD31 (red). Scale bar: 1000 μm. H) Flow cytometric analysis of mLECs percentage in NC group and rTHBS1 group, n = 4 per group, *** p < 0.001 versus NC by paired two-tailed Student’s t-test. I) Representative images of beads accumulation in dCLNs of the NC group and rTHBS1 group. Scale bar: 200 μm.

Human Csf Thbs1, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+csf+thbs1/Human+THBS1%2FTSP1%2FThrombospondin-1+ELISA+Kit+PicoKine/pm37211686-317-6-10
Average 92 stars, based on 3 article reviews

human csf thbs1 - by Bioz Stars, 2026-09

92/100 stars

Images

1) Product Images from "Single-Cell RNA Sequencing and Spatial Transcriptomics Reveal Pathogenesis of Meningeal Lymphatic Dysfunction after Experimental Subarachnoid Hemorrhage."

Article Title: Single-Cell RNA Sequencing and Spatial Transcriptomics Reveal Pathogenesis of Meningeal Lymphatic Dysfunction after Experimental Subarachnoid Hemorrhage.

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

doi: 10.1002/advs.202301428

Figure Legend Snippet: Figure 3. Intercellular communication networks in and around mLVs. A) Spatial visualization of immune cell inﬁltration based on mLECs distribution. B) Outgoing and incoming signal patterns among all meningeal cells. C) THBS signaling pathway network among all cell types. D) Spatial visualization of THBS1-CD47 L-R pair distribution by stLearn. E) Volcano plots showing that THBS1 is one of the upregulated DEGs in the SAH group compared to the NPH group identiﬁed by mass spectrometry. F) Quantiﬁcation of THBS1, THBS2, and THBS4 expression in CSF samples from SAH patients compared to those from NPH patients by ELISA assay, *** p < 0.001 versus NPH by paired two-tailed Student’s t-test. G) Representative confocal images of mLVs region of the negative control (NC) group and recombinant THBS1 protein treatment (rTHBS1) group. An enlarged view of TS and SSS is shown on the right in each group. Lyve1 (blue), Beads (green), and CD31 (red). Scale bar: 1000 μm. H) Flow cytometric analysis of mLECs percentage in NC group and rTHBS1 group, n = 4 per group, *** p < 0.001 versus NC by paired two-tailed Student’s t-test. I) Representative images of beads accumulation in dCLNs of the NC group and rTHBS1 group. Scale bar: 200 μm.

Techniques Used: Mass Spectrometry, Expressing, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Negative Control, Recombinant

Figure 4. Validation of THBS1 impacting mLVs function after SAH. SAH modeling and behavioral tests were performed 4 weeks after the AAV injection. A) Flow cytometric analysis of mLECs percentage in Sham, AAV-control, and AAV-THBS1 group at 72 h post-SAH, n = 4 per group, **p < 0.01, ***p < 0.001 by paired two-tailed Student’s t-test. B) Representative images and quantiﬁcation of beads accumulation in dCLNs of Sham, AAV-control, and AAV-THBS1 group at 72 h post-SAH, each data point represents an average of the 2 dCLNs from one individual mouse, n = 8 per group, **p < 0.01, ***p < 0.001 by paired two-tailed Student’s t-test. Scale bar: 200 μm. C) Representative confocal images of mLVs region of Sham, AAV-control, and AAV-THBS1 group at 72 h post-SAH. An enlarged view of TS and SSS is shown on the right in each group. Lyve1 (blue), Beads (green), and CD31 (red). Scale bar: 1000 μm. D) Modiﬁed Garcia test, E) time turn, F) time total, and G) wire hanging test at 72 h after SAH revealed AAV-THBS1 delivery aggravated short-term neurological function compared with Sham or AAV-control group, n = 10–12 per group. *p < 0.05; **p < 0.01, ***p < 0.001 by paired two-tailed Student’s t-test. H) Flow cytometric analysis of mLECs percentage in Sham, SAH-WT, and THBS1-KO group at 24 h post-SAH, n = 4 per group, **p < 0.01, ***p < 0.001 by paired two-tailed Student’s t-test. I) Representative images and quantiﬁcation of beads accumulation in dCLNs in sham, SAH-WT, and THBS-KO group at 24 h post-SAH, each data point represents an average of the 2 dCLNs from one individual mouse. n = 8 per group, **p < 0.01 by paired two-tailed Student’s t-test. Scale bar: 200 μm. J) Representative confocal images of mLVs region of Sham, SAH-WT, and THBS1-KO group at 24 h post-SAH. An enlarged view of TS and SSS is shown on the right in each group. Lyve1 (blue), Beads (green), and CD31 (red). Scale bar: 1000 μm. K) Modiﬁed Garcia test, L) time turn, M) time total, and N) wire hanging test at 24 h after SAH revealed THBS1 knockout improved short-term neurological function compared with Sham or WT-SAH group. n = 10–12 per group. *p < 0.05; **p < 0.01, ***p < 0.001 by paired two-tailed Student’s t-test.

Figure Legend Snippet: Figure 4. Validation of THBS1 impacting mLVs function after SAH. SAH modeling and behavioral tests were performed 4 weeks after the AAV injection. A) Flow cytometric analysis of mLECs percentage in Sham, AAV-control, and AAV-THBS1 group at 72 h post-SAH, n = 4 per group, **p < 0.01, ***p < 0.001 by paired two-tailed Student’s t-test. B) Representative images and quantiﬁcation of beads accumulation in dCLNs of Sham, AAV-control, and AAV-THBS1 group at 72 h post-SAH, each data point represents an average of the 2 dCLNs from one individual mouse, n = 8 per group, **p < 0.01, ***p < 0.001 by paired two-tailed Student’s t-test. Scale bar: 200 μm. C) Representative confocal images of mLVs region of Sham, AAV-control, and AAV-THBS1 group at 72 h post-SAH. An enlarged view of TS and SSS is shown on the right in each group. Lyve1 (blue), Beads (green), and CD31 (red). Scale bar: 1000 μm. D) Modiﬁed Garcia test, E) time turn, F) time total, and G) wire hanging test at 72 h after SAH revealed AAV-THBS1 delivery aggravated short-term neurological function compared with Sham or AAV-control group, n = 10–12 per group. *p < 0.05; **p < 0.01, ***p < 0.001 by paired two-tailed Student’s t-test. H) Flow cytometric analysis of mLECs percentage in Sham, SAH-WT, and THBS1-KO group at 24 h post-SAH, n = 4 per group, **p < 0.01, ***p < 0.001 by paired two-tailed Student’s t-test. I) Representative images and quantiﬁcation of beads accumulation in dCLNs in sham, SAH-WT, and THBS-KO group at 24 h post-SAH, each data point represents an average of the 2 dCLNs from one individual mouse. n = 8 per group, **p < 0.01 by paired two-tailed Student’s t-test. Scale bar: 200 μm. J) Representative confocal images of mLVs region of Sham, SAH-WT, and THBS1-KO group at 24 h post-SAH. An enlarged view of TS and SSS is shown on the right in each group. Lyve1 (blue), Beads (green), and CD31 (red). Scale bar: 1000 μm. K) Modiﬁed Garcia test, L) time turn, M) time total, and N) wire hanging test at 24 h after SAH revealed THBS1 knockout improved short-term neurological function compared with Sham or WT-SAH group. n = 10–12 per group. *p < 0.05; **p < 0.01, ***p < 0.001 by paired two-tailed Student’s t-test.

Techniques Used: Biomarker Discovery, Injection, Control, Two Tailed Test, Knock-Out

Figure 5. Disturbing THBS1-CD47 interaction promoted mLVs restoration via inhibiting STAT3/BCL2-mediated apoptosis in mLECs. A) Flow cytometric analysis of mLECs percentage in sham, SAH + igG, SAH + anti-CD47, and SAH + anti-THBS1 group at 24 h post-SAH, n = 4 per group, ***p < 0.001 by paired two-tailed Student’s t-test. B) Representative images and quantiﬁcation of beads accumulation in dCLNs of Sham, SAH + igG, SAH + anti-CD47, and SAH + anti-THBS1 group at 24 h post-SAH, each data point represents an average of the 2 dCLNs from one individual mouse, n = 8 per group, ***p < 0.001 by paired two-tailed Student’s t-test. Scale bar: 200 μm. C) Representative confocal images of mLVs region of Sham, SAH + igG, SAH + anti-CD47, and SAH + anti-THBS1 group at 24 h post-SAH. An enlarged view of TS and SSS is shown on the right in each group. Lyve1 (blue), Beads (green), and CD31 (red). Scale bar: 1000 μm. D) Modiﬁed Garcia test, E) time turn, F) time total, and G) wire hanging test at 24 h after SAH revealed anti-CD47 and anti-THBS1 therapy improved short-term neurological function compared with Sham or SAH + igG group. n = 10–12 per group. *p < 0.05; **p < 0.01, ***p < 0.001 by paired two-tailed Student’s t-test. H) GSEA showed apoptosis pathway was activated in mLECs after SAH. I) Representative immunoblot images showing eﬀects of rTHBS1 (100 ng mL−1) treatment on pSTAT3 and Bcl-2 inhibition in primary mLECs. J–M) Representative immunoblot images of STAT3, pSTAT3, Bax, and Bcl-2 in diﬀerent groups.

Figure Legend Snippet: Figure 5. Disturbing THBS1-CD47 interaction promoted mLVs restoration via inhibiting STAT3/BCL2-mediated apoptosis in mLECs. A) Flow cytometric analysis of mLECs percentage in sham, SAH + igG, SAH + anti-CD47, and SAH + anti-THBS1 group at 24 h post-SAH, n = 4 per group, ***p < 0.001 by paired two-tailed Student’s t-test. B) Representative images and quantiﬁcation of beads accumulation in dCLNs of Sham, SAH + igG, SAH + anti-CD47, and SAH + anti-THBS1 group at 24 h post-SAH, each data point represents an average of the 2 dCLNs from one individual mouse, n = 8 per group, ***p < 0.001 by paired two-tailed Student’s t-test. Scale bar: 200 μm. C) Representative confocal images of mLVs region of Sham, SAH + igG, SAH + anti-CD47, and SAH + anti-THBS1 group at 24 h post-SAH. An enlarged view of TS and SSS is shown on the right in each group. Lyve1 (blue), Beads (green), and CD31 (red). Scale bar: 1000 μm. D) Modiﬁed Garcia test, E) time turn, F) time total, and G) wire hanging test at 24 h after SAH revealed anti-CD47 and anti-THBS1 therapy improved short-term neurological function compared with Sham or SAH + igG group. n = 10–12 per group. *p < 0.05; **p < 0.01, ***p < 0.001 by paired two-tailed Student’s t-test. H) GSEA showed apoptosis pathway was activated in mLECs after SAH. I) Representative immunoblot images showing eﬀects of rTHBS1 (100 ng mL−1) treatment on pSTAT3 and Bcl-2 inhibition in primary mLECs. J–M) Representative immunoblot images of STAT3, pSTAT3, Bax, and Bcl-2 in diﬀerent groups.

Techniques Used: Two Tailed Test, Western Blot, Inhibition

Figure 6. Cell trajectory analysis shows the evolution of mLECs. A) Labeling mLECs with real grouping information based on pseudotime trajectory map. B) Pseudotime trajectory map. C) Trajectory of representative genes in mLECs. D) Heatmap showing the top 30 pseudotime-related genes. E) Representative two pseudo-time-related genes (S100𝛼6 and Cldn5) based on q values. F,G) Increased expression of THBS1 and S100A6 are associated with poor prognosis, n = 48, *p < 0.05 by paired two-tailed Student’s t-test. H) Linear relationship between THBS1 and S100A6 expression, r = 0.3779, p = 0.0081 by Spearman’s rank test. H) Representative confocal images of mLVs region of sham and SAH 24 h group. Enlarged view of selected region in the merged photo (yellow dotted box) are listed on the right in each group. Lyve1 (blue), S100𝛼6 (green), and CD31 (red). Yellow arrows indicate where mLVs lie. Scale bar: 800 μm for the holistic view and 100 μm for the enlarged view.

Figure Legend Snippet: Figure 6. Cell trajectory analysis shows the evolution of mLECs. A) Labeling mLECs with real grouping information based on pseudotime trajectory map. B) Pseudotime trajectory map. C) Trajectory of representative genes in mLECs. D) Heatmap showing the top 30 pseudotime-related genes. E) Representative two pseudo-time-related genes (S100𝛼6 and Cldn5) based on q values. F,G) Increased expression of THBS1 and S100A6 are associated with poor prognosis, n = 48, *p < 0.05 by paired two-tailed Student’s t-test. H) Linear relationship between THBS1 and S100A6 expression, r = 0.3779, p = 0.0081 by Spearman’s rank test. H) Representative confocal images of mLVs region of sham and SAH 24 h group. Enlarged view of selected region in the merged photo (yellow dotted box) are listed on the right in each group. Lyve1 (blue), S100𝛼6 (green), and CD31 (red). Yellow arrows indicate where mLVs lie. Scale bar: 800 μm for the holistic view and 100 μm for the enlarged view.

Techniques Used: Labeling, Expressing, Two Tailed Test

Enzyme-linked Immunosorbent Assay:

Article Title: Single-Cell RNA Sequencing and Spatial Transcriptomics Reveal Pathogenesis of Meningeal Lymphatic Dysfunction after Experimental Subarachnoid Hemorrhage.
Article Snippet: .. [52] ELISA Assay: The concentrations of human CSF THBS1 (EK0899, Boster Biotech, Wuhan), THBS2 (EK0642, Boster Biotech, Wuhan), THBS4 (CSB-EL023490HU, Cusabio Biotech, Wuhan), and S100A6 (CSBEL020634RA, Cusabio Biotech, Wuhan) were determined using ELISA kits. ..

Article Title: Single‐Cell RNA Sequencing and Spatial Transcriptomics Reveal Pathogenesis of Meningeal Lymphatic Dysfunction after Experimental Subarachnoid Hemorrhage
Article Snippet: .. The concentrations of human CSF THBS1 (EK0899, Boster Biotech, Wuhan), THBS2 (EK0642, Boster Biotech, Wuhan), THBS4 (CSB‐EL023490HU, Cusabio Biotech, Wuhan), and S100A6 (CSB‐EL020634RA, Cusabio Biotech, Wuhan) were determined using ELISA kits. ..

Clinical Proteomics:

Image Search Results

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Single-Cell RNA Sequencing and Spatial Transcriptomics Reveal Pathogenesis of Meningeal Lymphatic Dysfunction after Experimental Subarachnoid Hemorrhage.

doi: 10.1002/advs.202301428

Figure Lengend Snippet: Figure 3. Intercellular communication networks in and around mLVs. A) Spatial visualization of immune cell inﬁltration based on mLECs distribution. B) Outgoing and incoming signal patterns among all meningeal cells. C) THBS signaling pathway network among all cell types. D) Spatial visualization of THBS1-CD47 L-R pair distribution by stLearn. E) Volcano plots showing that THBS1 is one of the upregulated DEGs in the SAH group compared to the NPH group identiﬁed by mass spectrometry. F) Quantiﬁcation of THBS1, THBS2, and THBS4 expression in CSF samples from SAH patients compared to those from NPH patients by ELISA assay, *** p < 0.001 versus NPH by paired two-tailed Student’s t-test. G) Representative confocal images of mLVs region of the negative control (NC) group and recombinant THBS1 protein treatment (rTHBS1) group. An enlarged view of TS and SSS is shown on the right in each group. Lyve1 (blue), Beads (green), and CD31 (red). Scale bar: 1000 μm. H) Flow cytometric analysis of mLECs percentage in NC group and rTHBS1 group, n = 4 per group, *** p < 0.001 versus NC by paired two-tailed Student’s t-test. I) Representative images of beads accumulation in dCLNs of the NC group and rTHBS1 group. Scale bar: 200 μm.

Article Snippet: [52] ELISA Assay: The concentrations of human CSF THBS1 (EK0899, Boster Biotech, Wuhan), THBS2 (EK0642, Boster Biotech, Wuhan), THBS4 (CSB-EL023490HU, Cusabio Biotech, Wuhan), and S100A6 (CSBEL020634RA, Cusabio Biotech, Wuhan) were determined using ELISA kits.

Techniques: Mass Spectrometry, Expressing, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Negative Control, Recombinant

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Single-Cell RNA Sequencing and Spatial Transcriptomics Reveal Pathogenesis of Meningeal Lymphatic Dysfunction after Experimental Subarachnoid Hemorrhage.

doi: 10.1002/advs.202301428

Figure Lengend Snippet: Figure 4. Validation of THBS1 impacting mLVs function after SAH. SAH modeling and behavioral tests were performed 4 weeks after the AAV injection. A) Flow cytometric analysis of mLECs percentage in Sham, AAV-control, and AAV-THBS1 group at 72 h post-SAH, n = 4 per group, **p < 0.01, ***p < 0.001 by paired two-tailed Student’s t-test. B) Representative images and quantiﬁcation of beads accumulation in dCLNs of Sham, AAV-control, and AAV-THBS1 group at 72 h post-SAH, each data point represents an average of the 2 dCLNs from one individual mouse, n = 8 per group, **p < 0.01, ***p < 0.001 by paired two-tailed Student’s t-test. Scale bar: 200 μm. C) Representative confocal images of mLVs region of Sham, AAV-control, and AAV-THBS1 group at 72 h post-SAH. An enlarged view of TS and SSS is shown on the right in each group. Lyve1 (blue), Beads (green), and CD31 (red). Scale bar: 1000 μm. D) Modiﬁed Garcia test, E) time turn, F) time total, and G) wire hanging test at 72 h after SAH revealed AAV-THBS1 delivery aggravated short-term neurological function compared with Sham or AAV-control group, n = 10–12 per group. *p < 0.05; **p < 0.01, ***p < 0.001 by paired two-tailed Student’s t-test. H) Flow cytometric analysis of mLECs percentage in Sham, SAH-WT, and THBS1-KO group at 24 h post-SAH, n = 4 per group, **p < 0.01, ***p < 0.001 by paired two-tailed Student’s t-test. I) Representative images and quantiﬁcation of beads accumulation in dCLNs in sham, SAH-WT, and THBS-KO group at 24 h post-SAH, each data point represents an average of the 2 dCLNs from one individual mouse. n = 8 per group, **p < 0.01 by paired two-tailed Student’s t-test. Scale bar: 200 μm. J) Representative confocal images of mLVs region of Sham, SAH-WT, and THBS1-KO group at 24 h post-SAH. An enlarged view of TS and SSS is shown on the right in each group. Lyve1 (blue), Beads (green), and CD31 (red). Scale bar: 1000 μm. K) Modiﬁed Garcia test, L) time turn, M) time total, and N) wire hanging test at 24 h after SAH revealed THBS1 knockout improved short-term neurological function compared with Sham or WT-SAH group. n = 10–12 per group. *p < 0.05; **p < 0.01, ***p < 0.001 by paired two-tailed Student’s t-test.

Techniques: Biomarker Discovery, Injection, Control, Two Tailed Test, Knock-Out

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Single-Cell RNA Sequencing and Spatial Transcriptomics Reveal Pathogenesis of Meningeal Lymphatic Dysfunction after Experimental Subarachnoid Hemorrhage.

doi: 10.1002/advs.202301428

Figure Lengend Snippet: Figure 5. Disturbing THBS1-CD47 interaction promoted mLVs restoration via inhibiting STAT3/BCL2-mediated apoptosis in mLECs. A) Flow cytometric analysis of mLECs percentage in sham, SAH + igG, SAH + anti-CD47, and SAH + anti-THBS1 group at 24 h post-SAH, n = 4 per group, ***p < 0.001 by paired two-tailed Student’s t-test. B) Representative images and quantiﬁcation of beads accumulation in dCLNs of Sham, SAH + igG, SAH + anti-CD47, and SAH + anti-THBS1 group at 24 h post-SAH, each data point represents an average of the 2 dCLNs from one individual mouse, n = 8 per group, ***p < 0.001 by paired two-tailed Student’s t-test. Scale bar: 200 μm. C) Representative confocal images of mLVs region of Sham, SAH + igG, SAH + anti-CD47, and SAH + anti-THBS1 group at 24 h post-SAH. An enlarged view of TS and SSS is shown on the right in each group. Lyve1 (blue), Beads (green), and CD31 (red). Scale bar: 1000 μm. D) Modiﬁed Garcia test, E) time turn, F) time total, and G) wire hanging test at 24 h after SAH revealed anti-CD47 and anti-THBS1 therapy improved short-term neurological function compared with Sham or SAH + igG group. n = 10–12 per group. *p < 0.05; **p < 0.01, ***p < 0.001 by paired two-tailed Student’s t-test. H) GSEA showed apoptosis pathway was activated in mLECs after SAH. I) Representative immunoblot images showing eﬀects of rTHBS1 (100 ng mL−1) treatment on pSTAT3 and Bcl-2 inhibition in primary mLECs. J–M) Representative immunoblot images of STAT3, pSTAT3, Bax, and Bcl-2 in diﬀerent groups.

Techniques: Two Tailed Test, Western Blot, Inhibition

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Single-Cell RNA Sequencing and Spatial Transcriptomics Reveal Pathogenesis of Meningeal Lymphatic Dysfunction after Experimental Subarachnoid Hemorrhage.

doi: 10.1002/advs.202301428

Figure Lengend Snippet: Figure 6. Cell trajectory analysis shows the evolution of mLECs. A) Labeling mLECs with real grouping information based on pseudotime trajectory map. B) Pseudotime trajectory map. C) Trajectory of representative genes in mLECs. D) Heatmap showing the top 30 pseudotime-related genes. E) Representative two pseudo-time-related genes (S100𝛼6 and Cldn5) based on q values. F,G) Increased expression of THBS1 and S100A6 are associated with poor prognosis, n = 48, *p < 0.05 by paired two-tailed Student’s t-test. H) Linear relationship between THBS1 and S100A6 expression, r = 0.3779, p = 0.0081 by Spearman’s rank test. H) Representative confocal images of mLVs region of sham and SAH 24 h group. Enlarged view of selected region in the merged photo (yellow dotted box) are listed on the right in each group. Lyve1 (blue), S100𝛼6 (green), and CD31 (red). Yellow arrows indicate where mLVs lie. Scale bar: 800 μm for the holistic view and 100 μm for the enlarged view.

Techniques: Labeling, Expressing, Two Tailed Test

Review

human csf thbs1 (Boster Bio)

Structured Review

Images

1) Product Images from "Single-Cell RNA Sequencing and Spatial Transcriptomics Reveal Pathogenesis of Meningeal Lymphatic Dysfunction after Experimental Subarachnoid Hemorrhage."

Related Articles

Enzyme-linked Immunosorbent Assay:

Clinical Proteomics:

Similar Products

Image Search Results